ITGA2 promotes expression of ACLY and CCND1 in enhancing breast cancer stemness and metastasis

Cancer metastasis is largely incurable and accounts for 90% of breast cancer deaths, especially the aggressive basal-like or triple negative (TNBC). Combining patient database analyses functional studies, we examined association integrin family members with clinical outcomes as well their connection previously identified microRNA regulators metastasis, such miR-206 that inhibits stemness TNBC. Here report receptor CD49b-encoding ITGA2 , a direct target miR-206, promotes metastasis. knockdown suppressed self-renewal related mammosphere formation pluripotency marker expression, inhibited cell cycling, compromised migration invasion, therefore decreased lung cancer. overexpression reversed miR-206-caused cycle arrest in G1. RNA sequencing revealed genes to regulation lipid metabolism, including CCND1 ACLY representative targets, respectively. Knockdown cells. Overexpression rescues phenotype knockdown-induced arrest. -encoded ATP citrate lyase essential maintain cellular acetyl-CoA levels. further mimics abolishing colonization We low levels high expression are associated an unfavorable relapse-free survival patients estrogen receptor-negative grade cancer, TNBC, possibly serving potential biomarkers therapeutic targets